Vigilancia Tecnológica

Changes in the composition of the EPS produced by L. amnigena in different culture media and irradiated by Laser or LED light sources and studied using Raman spectroscopy showed peaks of fucose, glucose, galactose, glucuronic acid and mannose. PCA showed the presence of acyl groups (mainly pyruvyl) depending on the production conditions and irradiation.ABSTRACTThis study aimed to compare the changes in the composition of the exopolysaccharide (EPS) produced by Lelliottia amnigena in culture medium containing distilled water (DW) and dialyzed produced water (DPW) irradiated by either Laser (?660 nm, 8.0?J/cm2) or LED (?630 nm, 12.0?J/cm2) during bacterial growth using Raman spectroscopy at 1064?nm. The cultures of L. amnigena were irradiated at 9? and 12?h, and the EPS obtained from different production protocols were analyzed dehydrated. Raman spectra showed peaks assigned to saccharides from EPS polymer, and principal component analysis revealed differences in the composition of the EPS produced depending on the water used in production and the light source used for irradiation. Remarkably, the presence of acyl groups (acetyl and pyruvyl) in the mannose residues at the group DW and mannose without evidence of acetyl in the irradiated groups; the irradiated groups also presented evidence of carboxylate (succinyl).